abstract：:We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to ...

journal_title：Molecular immunology

authors： Stanker LH,Wyrobek A,McKeown C,Balhorn R

更新日期：1993-12-01 00:00:00

abstract：:The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD....

journal_title：Molecular immunology

authors： Helm R,Cockrell G,Sharkey P,Brenner R,Burks AW

更新日期：1993-12-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF) has been implicated in the pathogenesis of a variety of human diseases including septic shock, cachexia, graft-versus-host disease and several autoimmune diseases. Monoclonal antibodies directed against TNF provide an attractive mode of therapeutic intervention in these diseases. We h...

journal_title：Molecular immunology

authors： Knight DM,Trinh H,Le J,Siegel S,Shealy D,McDonough M,Scallon B,Moore MA,Vilcek J,Daddona P

更新日期：1993-11-01 00:00:00

abstract：:Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We com...

journal_title：Molecular immunology

authors： Klaus SJ,Phillips NE,Parker DC

更新日期：1993-11-01 00:00:00

abstract：:We have recently isolated and characterized human and mouse genes of a putative natural killer (NK) cell tumour-recognition protein (NK-TR) that is specifically expressed in NK cells. This gene codes for a 150 kD protein with a cyclophilin-related amino terminus followed by several positively charged domains. We repor...

journal_title：Molecular immunology

authors： Rinfret A,Anderson SK

更新日期：1993-10-01 00:00:00

abstract：:Factor I (C3b/C4b inactivator) is a regulatory protein of the classical and alternative complement pathways. In this paper, we report the sequence of Xenopus factor I cDNA and the deduced protein structure. The basic structure of human preprofactor I, NH2-heavy chain-cleavage peptide-light chain-COOH, is conserved in ...

journal_title：Molecular immunology

authors： Kunnath-Muglia LM,Chang GH,Sim RB,Day AJ,Ezekowitz RA

更新日期：1993-10-01 00:00:00

abstract：:Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-ce...

journal_title：Molecular immunology

authors： Chen YH,Böck G,Vornhagen R,Steindl F,Katinger H,Dierich MP

更新日期：1993-09-01 00:00:00

abstract：:A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

journal_title：Molecular immunology

authors： Devaux C,Juin M,Mansuelle P,Granier C

更新日期：1993-08-01 00:00:00

abstract：:Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...

journal_title：Molecular immunology

authors： Weidner KM,Denzin LK,Kim ML,Mallender WD,Miklasz SD,Voss EW Jr

更新日期：1993-08-01 00:00:00

abstract：:Electron microscopy of specimens of C9 tilted through 90 degrees visualized this protein to be a globular ellipsoid with dimensions of 77 x 70 x 52 A. To check the congruence of this observation with physical properties of the molecule, hydrodynamic parameters for C9 were determined. From this work a frictional ratio ...

journal_title：Molecular immunology

authors： DiScipio RG

更新日期：1993-08-01 00:00:00

abstract：:Covalent Superose microspheres-bound C3b was used as a model system to simplify the analysis of antigen-bound C3b modifications during antigen processing. The model was set up using purified C3 and Superose-bound trypsin. C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxyla...

journal_title：Molecular immunology

authors： Rey-Millet CA,Chesne S,Colomb MG

更新日期：1993-07-01 00:00:00

abstract：:Human cell lines and blood lymphocytes were treated for short time periods with IFN-gamma. This treatment increased the amount of the assembled MHC class I molecules on the plasma membrane after 30 min. This early increase of the membrane expression subsided in the next few hours. A second wave of elevation occurred a...

journal_title：Molecular immunology

authors： Végh Z,Wang P,Vánky F,Klein E

更新日期：1993-06-01 00:00:00

abstract：:Immunization of different mice strains with a recombinant fusion protein composed of the vector-encoded N-terminal leader peptide CroLac (containing lambda Cro and LacI fragments) and a part of the transmembrane protein of HIV-1 (gp41) led to a high anti-CroLac humoral immune response. A detailed analysis of this resp...

journal_title：Molecular immunology

authors： Scheerlinck JP,DeLeys R,Saman E,Brys L,Geldhof A,De Baetselier P

更新日期：1993-06-01 00:00:00

abstract：:C-reactive protein (CRP) binds to chromatin, histones, and small nuclear ribonucleoproteins (snRNPs) through a phosphocholine (PC)-inhibitable, calcium-dependent binding site. snRNPs process pre-mRNA to mature mRNA and are composed of small uridine-rich RNAs (designated U1, U2, U5 and U4/U6) and associated proteins. W...

journal_title：Molecular immunology

authors： Jewell WS,Marnell LL,Rokeach LA,Du Clos TW

更新日期：1993-06-01 00:00:00

abstract：:Structural similarities between two members of the immunoglobulin superfamily were explored by making chimeric immunoglobulin/CD4 antigen molecules. A crossover in the middle of the originally proposed J kappa homology unit of the first domain of the CD4 molecule was used to construct a chimeric molecule having human ...

journal_title：Molecular immunology

authors： Frey T,Estess P,Oi VT

更新日期：1993-06-01 00:00:00

abstract：:A turbidimetric method for measuring the diameters of insoluble immune complexes, based on the wavelength dependence of their ability to scatter light, was developed. The method was validated by demonstrating that it gave experimental values for the diameters of polystyrene microspheres which were in good agreement wi...

journal_title：Molecular immunology

authors： Easterbrook-Smith SB

更新日期：1993-05-01 00:00:00

abstract：:The functional importance of each amino acid residue making up the antigenic determinants of three different peptide-mAb interactions was determined using complete series of substitution analogs of the three immunizing synthetic peptides. Fingerprint substitution profiles for the three different antigenic determinants...

journal_title：Molecular immunology

authors： Pinilla C,Appel JR,Houghten RA

更新日期：1993-04-01 00:00:00

abstract：:The antigenicity of Human Immunodeficiency Virus type 1 (HIV-1) matrix p18 protein was evaluated by analyzing the specificity of anti-p18 antibodies elicited either in HIV-1 infected humans, or in HIV-1 infected or immunized chimpanzees, against a panel of long and short overlapping synthetic peptides [from 12 to 46 a...

journal_title：Molecular immunology

authors： Mabrouk K,Moulard M,Gluckman JC,Romi R,Rochat H,Van Rietschoten J,Bahraoui E

更新日期：1993-04-01 00:00:00

abstract：:Phosphocholine (PC)-specific antibodies that arise in the mouse in response to Proteus morganii (PM) and use V1-DFL16.1-JH1 are characterized by a number of recurring mutations. Most striking is an invariant A for G substitution in codon 95 of VH which results in an asparagine instead of aspartate at that position. Be...

journal_title：Molecular immunology

authors： George J,Sheehan KM,Brodeur PH,Claflin JL

更新日期：1993-03-01 00:00:00

abstract：:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...

journal_title：Molecular immunology

authors： LaCroix EL,Froese A

更新日期：1993-02-01 00:00:00

abstract：:Heparin and two dextran sulphate preparations with a low or high average molecular mass (M(r) 5000 and 5 x 10(5), respectively) enhanced binding of radioactively labelled complement factor H to the complement protein C3b, coupled to Sepharose 4B, maximally 2.5-4-fold within a polyanion concn range of 12.5-400 microgra...

journal_title：Molecular immunology

authors： Koistinen V

更新日期：1993-02-01 00:00:00

abstract：:This study describes serological and biochemical properties of a novel MHC class I molecule. The mutant H-2Ksm1 molecule was discovered in a mouse because of loss of reactivity of its peripheral blood lymphocytes to monoclonal antibodies. This mutation in the H-2Ks molecule is the first in vivo mutation described that...

journal_title：Molecular immunology

authors： Hasenkrug KJ,Kesari KV,Nathenson SG

更新日期：1992-12-01 00:00:00

abstract：:The fifth component of the complement cascade, C5a, was iodinated using the Bolton-Hunter reagent. Results from the present study, using the high affinity ligand, [125I]Bolton-Hunter-labeled C5a ([125I]BH-C5a), revealed a single binding site on membranes prepared from human neutrophils, U-937 cells and human monocytes...

journal_title：Molecular immunology

authors： Braunwalder AF,Musmanno D,Galakatos N,Garlick RH,Haston WO,Rediske JJ,Wennogle L,Seligmann B,Sills MA

更新日期：1992-11-01 00:00:00

abstract：:Ovalbumin (OVA) is a major allergen (Gal d II) of hen egg white and is often the cause of hypersensitivity reactions to food. Further knowledge of the antigenic and allergenic epitopes of allergens will provide better treatment of this disease. To analyse these epitopes we produced a panel of monoclonal antibodies (mA...

journal_title：Molecular immunology

authors： Kahlert H,Petersen A,Becker WM,Schlaak M

更新日期：1992-10-01 00:00:00

abstract：:Ig production by splenic human B cells that express different surface Ig isotypes were analysed in limiting dilution cultures. Therefore, FACS sorted IgM+, IgG+ and IgA1+ B cells were stimulated with PMA-activated EL4 thymoma cells as helper cells in the presence of IL-2 and IL-4. We found that at least every second B...

journal_title：Molecular immunology

authors： Brinkmann V,Müller S,Heusser CH

更新日期：1992-10-01 00:00:00

abstract：:We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region s...

journal_title：Molecular immunology

authors： Wu TT,Kabat EA

更新日期：1992-09-01 00:00:00

abstract：:The characterization of the site on the IgE molecule which accommodates the high affinity receptor for IgE (Fc epsilon RI) should allow the design of IgE analogues which can be utilized to block allergic responses. Using chimeric human IgE molecules in which different constant region domains were exchanged with their ...

journal_title：Molecular immunology

authors： Nissim A,Eshhar Z

更新日期：1992-09-01 00:00:00

abstract：:The variables regions of three syngeneic anti-idiotypic antibodies (Ab2s) were cloned and sequenced. They are encoded by different VL genes, two are from different members of V kappa-Ox1 superfamily. The H chains are encoded by VH genes belonging to three different VH families, J558, Q52 and 7183. Together with a prev...

journal_title：Molecular immunology

authors： Chen J,Borden P,Liao J,Kabat EA

更新日期：1992-09-01 00:00:00

abstract：:Immature B cell lines transformed with a temperature-sensitive mutant of Abelson murine leukaemia virus undergo preferentially V gamma 2 to J gamma 2 joinings during culture at a non-permissive temperature (39 degrees C). Here we examined nucleotide addition and deletion at the V gamma 2-J gamma 2 junctions in these V...

journal_title：Molecular immunology

authors： Tsukada S,Oka Y,Sugiyama H

更新日期：1992-07-01 00:00:00

abstract：:A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...

journal_title：Molecular immunology

authors： Patri S,Nau F

更新日期：1992-07-01 00:00:00

abstract：:The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 prove...

journal_title：Molecular immunology

authors： Kontsek P,Borecký L,Kontseková E,Kolcunová A,Novák M,Zav'yalov VP,Maiorov VA

更新日期：1992-07-01 00:00:00

abstract：:We have recently reported cellular growth arrest induced following crosslinking of surface IgM (sIgM) but not surface IgD (sIgD) in the WEHI-231 cell line, representative of the immature B cell stage, and its delta heavy chain (delta) transfectant. An initial report has indicated WEHI-231.7, a subclone of WEHI-231, fa...

journal_title：Molecular immunology

authors： Iwabuchi N,Williams DB,Nguyen HP,Hozumi N

更新日期：1992-05-01 00:00:00

abstract：:The envelope protein of the feline immunodeficiency virus (FIV) was analyzed using several epitope prediction programs based on profiles of hydrophilicity, antigenicity, and probability of residues to lie on the protein surface. Tentative homologies with the immunodominant epitope sites in simian virus (SIV) or human ...

journal_title：Molecular immunology

authors： Avrameas A,Guillet JG,Chouchane L,Moraillon A,Sonigo P,Strosberg AD

更新日期：1992-05-01 00:00:00

abstract：:The expression and ligand binding activity of Fc receptors for IgG (Fc gamma R) on guinea-pig peripheral blood polymorphonuclear leukocytes (blood PMN) have been compared with those on casein-elicited peritoneal PMN (exudate PMN). Both the PMN were found to express two distinct types of Fc gamma R, one specific for Ig...

journal_title：Molecular immunology

authors： Fukuchi Y,Sato M,Yamashita T,Koyama J

更新日期：1992-05-01 00:00:00

abstract：:In human peripheral blood, most of the CD3+ cells express the alpha/beta T cell receptor. A smaller fraction of CD3+ T cells express the gamma/delta T cell receptor (from 1 to 10% depending the individuals, with an average of 3-5%). Interestingly, although the alpha/beta + T cells never express the gamma chain at the ...

journal_title：Molecular immunology

authors： Alexandre D,Lefranc MP

更新日期：1992-04-01 00:00:00

abstract：:The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...

journal_title：Molecular immunology

authors： Shaw SY,Margolies MN

更新日期：1992-04-01 00:00:00

abstract：:As an initial step towards the molecular analysis of the murine V kappa locus, a cosmid library from BALB/cJ mouse liver DNA was screened with probes representing 10 V kappa families. Of eight cosmids that were isolated from the initial screen, five contained a single restriction fragment that hybridized to the probes...

journal_title：Molecular immunology

authors： Lawler AM,Umar A,Gearhart PJ

更新日期：1992-03-01 00:00:00

abstract：:A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning membe...

journal_title：Molecular immunology

authors： Chao M,Voss EW Jr

更新日期：1992-03-01 00:00:00

abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...

journal_title：Molecular immunology

authors： Ghaffari SH,Lobb CJ

更新日期：1992-02-01 00:00:00

abstract：:In this paper, we performed further biochemical characterization of the CD26 antigen, as defined by the mAbs in anti-1F7 and anti-Ta1, in order to clarify the observed functional differences among these mAbs. For this purpose, we developed a mAb, anti-5F8, which recognizes yet another epitope on the CD26 antigen diffe...

journal_title：Molecular immunology

authors： Torimoto Y,Dang NH,Tanaka T,Prado C,Schlossman SF,Morimoto C

更新日期：1992-02-01 00:00:00